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FSEN1 sensitizes multiple cancer cell lines of different tissue origins to ferroptosis To examine the role of FSP1 in suppressing ferroptosis in different types of cancer, we measured the impact of FSEN1 on RSL3-induced cell death in a panel of cancer cell lines of various tissue origins, including lung (A549 N ), breast (HCC1143 C ), liver (Huh7 C ), glial (T98G N ), bone (U 2-OS N ), connective (HT-1080 N ), lymphoid (RL, SUDHL5), and skin (A375, SKMEL28, 501-MEL) (Figure 5A,B and Figure S5A,B)

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